IgE Monoclonal Antibody (Ige21), Super Bright™ 780, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 78-698-642-Each-of-1 | In Stock | ₹ 30,050.85 |
78-698-642 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 30,050.85
GST (18%): ₹ 5,409.153
Total Price: ₹ 35,460.003
Antigen
IgE
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
P01854
Gene Symbols
IGHE
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
3497
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
Ige21
Conjugate
Super Bright 780
Gene
IGHE
Gene Alias
HCAK1; IgA1; IgG3; IGHA1; IGHA2; IGHG1; IGHG2; IGHG3; IGHG4; IGKC; IGKCD; IGLC; IGLC1; Immunoglobulin E; immunoglobulin heavy constant alpha 1; immunoglobulin heavy constant alpha 2 (A2m marker); immunoglobulin heavy constant gamma 1 (G1m marker); immunoglobulin heavy constant gamma 2 (G2m marker); immunoglobulin heavy constant gamma 3 (G3m marker); immunoglobulin heavy constant gamma 4 (G4m marker); immunoglobulin kappa constant; immunoglobulin lambda constant 1; ImmunoglobulinE; Km
Host Species
Mouse
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1 κ
Related Products
Description
- Description: The 2D1 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA)
- CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets
- The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes
- Applications Reported: This 2D1 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This 2D1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- This may be used at 5 μL (0.5 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm
- We recommend using a 780/60 bandpass filter
- Please make sure that your instrument is capable of detecting this fluorochrome
- In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product No
- 01-2222-42) as compared to single-color stained cells
- In such circumstances, we would recommend setting compensation with cells
- IgE is one of the five classes of immunoglobulins found in mammals
- Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains
- IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells
- Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations
- IgE is also functionally important for immunity against parasites.