phospho Histone H4 (Tyr51), Rabbit anti-Human, Polyclonal, Millipore Sigma™
Manufacturer: MilliporeSigma
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABE290925UL-Each-of-1 | In Stock | ₹ 17,118.26 |
ABE290925UL - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,118.26
GST (18%): ₹ 3,081.287
Total Price: ₹ 20,199.547
Antigen
phospho Histone H4 (Tyr51)
Classification
Polyclonal
Dilution
Western Blotting Analysis: A representative lot detected Tyr51 phosphorylation of Histone H4 in the presence of active recombinant Tie2, following analysis by Western blotting. (Hossain, MB., et al. (2016). Sci. Adv. 2; e1501290). Immunofluorescence Analysis: A representative lot detected phospho Histone H4 (Tyr51) in HEK293 Tie2 cells treated with Angiopoietin 1 ( ANG1) and ionizing radiation (Hossain, MB., et al. (2016). Sci. Adv. 2; e1501290).
Gene Alias
H4;H4Y51;H4pY51
Host Species
Rabbit
Molecular Weight of Antigen
11.38 kDa calculated.
Quantity
25 μL
Research Discipline
Epigenetics & Nuclear Function
Test Specificity
This rabbit polyclonal antibody detects Histone H4 phosphorylated on Tyrosine 51. It targets an epitope within 11 amino acids surrounding phosphotyrosine 51.
Content And Storage
Stable for 1 year at -20°C from date of receipt.
Isotype
IgG
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Conjugate
Unconjugated
Formulation
Purified rabbit polyclonal antibody containing PBS without preservatives and 40% glycerol.
Gene Symbols
HIST14A;H4/A;H4FA;HIST1H4B;H4/I;H4FI;HIST1H4C;H4/G;H4FG;HIST1H4D;H4/B;H4FB;HIST1H4E;H4/J;H4FJ;HIST1H4F;H4/C;H4FC;HIST1H4H;H4/H;H4FH;HIST1H4I;H4/M;H4FM;HIST1H4J;H4/E;H4FE;HIST1H4K;H4/D;H4FD;HIST1H4L;H4/K;H4FK;HIST2H4A;H4/N;H4F2;H4FN;HIST2H4;HIST2H4B;H4/O;H4FO;HIST4H4
Immunogen
Two linear peptides (phosphorylated on Tyr 51 and non-phosphorylated) corresponding to 11 amino acids from the internal region of human Histone H4.
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Human
Form
Purified
Related Products
Description
- Anti-phospho Histone H4 (Tyr51) Antibody, Cat
- No
- ABE2909, is a highly specific rabbit polyclonal antibody that targets Histone H4 phosphorylated on Tyr51 and has been tested for use in in Immunocytochemistry, Immunofluorescence, and Western Blotting
- Histone H4 (UniProt: P62805 ) is encoded by the HIST14A (also known as H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4) gene (Gene ID: 121504, 554313, 8294, 8359, 8360, 8361, 8362, 8363, 8364, 8365, 8366, 8367, 8368, 8370) in human
- Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability
- DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling
- Histone H4 is one of the main histone proteins that contribute to the chromatin structure in both prokaryotic and eukaryotic cells
- Histone H4 can undergo several different types of epigenetic modifications that influence cellular processes, including methylation, acetylation, and phosphorylation
- Acetylation at Lys6, Lys9, Lys13 and Lys17 is shown to occur in coding regions of the genome but not in heterochromatin
- Monomethylation and asymmetric dimethylation at Arg4 is reported to favor acetylation at Lys9 and Lys13
- Histone H4 can be phosphorylated by Tie2 on Tyr51, which is shown to preferentially occur in the presence of core histones
- The phosphorylated H4 (H4Y51) is recognized by the proto-oncogene ABL1
- Histone H4 is also phosphorylated on Ser48 by PAK2, which is shown to increase the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4
- Chromosomal aberrations involving histone H4 causes of B-cell non-Hodgkin lymphomas
- (Ref.: Hossain, MB., et al
- (2016)
- Sci
- Adv
- 2; e1501290).