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ABF116925UL

Pyrin, Rabbit anti-Human, Polyclonal, Millipore Sigma™

Manufacturer: MilliporeSigma

Select a Size

Pack Size SKU Availability Price
Each of 1 ABF116925UL-Each-of-1 In Stock ₹ 17,118.26

ABF116925UL - Each of 1

₹ 17,118.26

In Stock

Quantity

1

Base Price: ₹ 17,118.26

GST (18%): ₹ 3,081.287

Total Price: ₹ 20,199.547

Antigen

Pyrin

Classification

Polyclonal

Dilution

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Pyrin in human tonsil, human cerebral cortex, human skeletal muscle, and human pancreatic cancer tissues.

Gene Alias

Marenostrin

Host Species

Rabbit

Molecular Weight of Antigen

∽82 kDa observed; 86.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Quantity

25 μL

Research Discipline

Inflammation & Immunology

Test Specificity

This rabbit polyclonal antibody detects Pyrin in human cells. It targets an epitope within 15 amino acids from the N-terminal region.

Content And Storage

Stable for 1 year at 2-8°C from date of receipt.

Applications

Immunohistochemistry (Paraffin), Western Blot

Conjugate

Unconjugated

Formulation

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Gene Symbols

MEFV;MEF;TRIM20

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Pyrin, isoform 1.

Purification Method

Affinity Purified

Regulatory Status

RUO

Primary or Secondary

Primary

Target Species

Human

Description

  • Anti-Pyrin, Cat
  • No
  • ABF1169, is a rabbit polyclonal antibody that detects human Pyrin and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting
  • Pyrin (UniProt: O15553; also known as Marenostrin) is encoded by the MEFV (also known as MEF, TRIM20) gene (Gene ID: 4210) in human
  • Pyrin is a homotrimeric protein that is involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma
  • It organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy
  • Pyrin acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence it may prevent excessive IL-1B- and IL-18-mediated inflammation
  • Pyrin is expressed in peripheral blood leukocytes, particularly in mature granulocytes and to a lesser extent in monocytes, but not in lymphocytes
  • It is also detected in spleen, lung and muscle, probably as a result of leukocyte infiltration in these tissues
  • Its expression is also detected in several myeloid leukemic, colon cancer, and prostate cancer cell lines
  • Three isoforms of Pyrin have been described that are produced by alternative splicing
  • Isoform 1 associates with microtubules and with the filamentous actin of perinuclear filaments and peripheral lamellar ruffles
  • In pre-apoptotic cells, it co-localizes with PYCARD/ASC in large specks (inflammasome)
  • Isoform 2 is shown to be localized to the nucleus.