MilliporeSigma™ HNE Michael Adducts, Rabbit, Unlabeled, Polyclonal,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABN249MI-Each-of-1 | In Stock | ₹ 43,318.08 |
ABN249MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 43,318.08
GST (18%): ₹ 7,797.254
Total Price: ₹ 51,115.334
Antigen
HNE Michael Adducts
Classification
Polyclonal
Conjugate
Unconjugated
Host Species
Rabbit
Purification Method
Unpurified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Bovine
Form
Serum
Applications
Western Blot
Concentration
Please refer to lot specific datasheet.
Formulation
Rabbit polyclonal antibody serum with 0.05% Sodium Azide.
Immunogen
HNE-modifed KLH chemically reduced by sodium borohydride.
Quantity
100 μL
Research Discipline
Neuroscience
Test Specificity
This polyclonal antiserum detected HNE-modified BSA only after, but not before, reduction by sodium borohydride.
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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Description
- Reactive aldehydes generated during lipid peroxidation contribute to cell and tissue damage associated with oxidative stress
- Alpha,beta-unsaturated carbonyl compounds such as 4-hydroxy-2-nonenal (HNE), 4-oxo-2-nonenal (ONE), and acrolein, generated from oxidation of polyunsaturated fatty acids (PUFAs), readily interact with nucleophilic groups on proteins, leading to intramolecular or intermolecular protein crosslinking
- Two types of HNE modifications exist, the first type is formed by a 1:1 Michael adduct reaction with lysine amine, histine imidazole, or cysteine sulfhydryl, while the second type is produced by one HNE reacting simultaneously with two free amine groups from two N-alpha-acetyllysines (NALs), so-called Michael-Schiff base adduct
- In the latter case, the initial nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base further undergoes oxidative cyclization to form a fluorescent 2-hydroxy-3-imino-1,2-dihydropyrrol derivative commonly referred to as HNE fluorophore
- Reversibly formed HNE-Lys Michael adduct is not stable and reduction by sodium borohydride (NaBH4) is often employed to preserve Lys HNE mofidication in protein samples
- Reductive stabilization by NaBH4 treatment is also used to prevent 4-HNE loss from 4-HNE-Cys Michael adduct by retro-Michael addition reaction (RMA) during collision-induced dissociation (CID) and electron transfer dissociation (ETD) MS/MS.