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ABS148625UG

E3 ubiquitin-protein ligase RNF31, Rabbit anti-Human, Polyclonal, Millipore Sigma™

Manufacturer: MilliporeSigma

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Pack Size SKU Availability Price
Each of 1 ABS148625UG-Each-of-1 In Stock ₹ 17,118.26

ABS148625UG - Each of 1

₹ 17,118.26

In Stock

Quantity

1

Base Price: ₹ 17,118.26

GST (18%): ₹ 3,081.287

Total Price: ₹ 20,199.547

Antigen

E3 ubiquitin-protein ligase RNF31

Classification

Polyclonal

Dilution

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected E3 ubiquitin-protein ligase RNF31 in human adrenal gland and human uterus tissues.

Gene Alias

EC: 2.3.2.27;HOIL-1-interacting protein;HOIP;RING finger protein 31;RING-type E3 ubiquitin transferase RNF31;Zinc in-between-RING-finger ubiquitin-associated domain protein

Host Species

Rabbit

Molecular Weight of Antigen

∽120 kDa observed; 119.65 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Quantity

25 μg

Research Discipline

Signaling

Test Specificity

This rabbit polyclonal antibody detects E3 ubiquitin-protein ligase RNF31 in human cells. It targets an epitope within 17 amino acids from the C-terminal region. Immunogen sequence is conserved in all 3 isoforms.

Content And Storage

Stable for 1 year at 2-8°C from date of receipt.

Applications

Immunohistochemistry (Paraffin), Western Blot

Conjugate

Unconjugated

Formulation

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Gene Symbols

RNF31;ZIBRA

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human E3 ubiquitin-protein ligase RNF31.

Purification Method

Affinity Purified

Regulatory Status

RUO

Primary or Secondary

Primary

Target Species

Human

Description

  • Anti-E3 ubiquitin-protein ligase RNF31, Cat
  • No
  • ABS1486, is a rabbit polyclonal antibody that detects E3 ubiquitin-protein ligase RNF31 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting
  • E3 ubiquitin-protein ligase RNF31 (UniProt: Q96EP0; also known as EC: 2.3.2.27, HOIL-1-interacting protein, HOIP, RING finger protein 31, RING-type E3 ubiquitin transferase RNF31, Zinc in-between-RING-finger ubiquitin-associated domain protein) is encoded by the RNF31 (also known as ZIBRA) gene (Gene ID: 55072) in human
  • E3 ubiquitin-protein ligase RNF31 is a cytoplasmic protein that is a component of the linear ubiquitin chain assembly complex (LUBAC complex), which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation
  • LUBAC conjugates linear polyubiquitin to IKBKG (NEMO) and receptor interacting serine/threonine Kinase 1 (RIPK1) and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways
  • LUBAC-mediated polyubiquitination interferes with TNF-induced cell death and thereby prevents inflammation
  • Together with OUTLIN deubiquitinase, the LUBAC complex is also reported to regulate the canonical Wnt signaling pathway during angiogenesis
  • E3 ubiquitin-protein ligase RNF31 is expressed in both normal and transformed breast epithelial cell lines
  • It contains a polyubiquitin-binding (PUB) domain (aa 71-142) that mediates interaction with the PCNA-interacting motifs of C-terminus of the AAA+ ATPase p97 (VCP) and RNF31, with a strong preference for RNF3
  • It also has a ubiquitin-associated (UBA) domain (aa 564-615) that mediates association with RanBP-type and C3HC4-type zinc finger-containing protein 1 (RBCK1/HOIL1) via interaction with its ubiquitin-like (UBL) domain
  • (Ref.: Elliot, PR et al (2014)
  • Mol
  • Cell 54(3); 335-48).