sCD14 Rat anti-Human, Unlabeled, Clone: 55-3, BD

Description

• The 55-3 monoclonal antibody reacts with human CD14
• CD14 is a 53 - 55kDa cell surface glycoprotein that is expressed by monocytes, macrophages, and activated granulocytes as a glycophosphatidylinositol-linked protein
• CD14 functions as a receptor for lipopolysaccharide (LPS, endotoxin) when LPS is bound to LBP (LPS binding protein)
• Soluble forms of CD14 (sCD14) can be detected either in serum or in supernatants from cell cultures
• Soluble CD14 is produced either by shedding of membrane CD14 or by release from cells before addition of the GPI anchor
• Both membrane and sCD14 function to enhance cell activation by LPS
• Binding of LPS to CD14 activates monocytes or macrophages to produce cytokines and express adhesive proteins and low molecular weight proinflammatory mediators
• Non-CD14 expressing cells, such as endothelial cells, can be activated by a complex of LPS and sCD14
• Serum levels of sCD14 increase in association with sepsis, infectious disease, autoimmunity, and allergic disorders
• The gene for human CD14 has been cloned and sequenced and recombinant sCD14 has been shown to be protective against LPS-induced lethality
• The immunogen used to generate the 55-3 hybridoma was purified, soluble recombinant human CD14 protein
• Host Species: Rat Clone: 55-3 Isotype: IgG2a κ Species Reactivity: Human Immunogen: Human CD14 Recombinant Protein ELISA