H2-M Rat anti-Mouse, Unlabeled, Clone: 2E5A, BD

Description

• H2-M, also known as H2-DM, is a non-classical MHC class II molecule in antigen-presenting cells
• H2-M and CD74 (Ii) are critical components of the antigen-processing pathway of the classical MHC class II molecules
• Like classical MHC class II molecules, non-classical MHC molecules have limited polymorphism
• There are two isoforms of H2-M, αββ1 and αβ2, encoded by the H2-DMa gene and either H2-DMb1 or H2-DMb2, respectively
• The 2E5A antibody reacts with H2-M αβ2 dimers (the predominant form transcribed in the mouse spleen), but not αβ1 dimers
• H2-M dimers (both isoforms) are integral proteins of lysomal membranes, where they catalyze the release of CLIP (class II-associated Ii peptide) from the peptide-binding groove of classical MHC class II dimers and stabilize the open binding site to allow loading of exogenous peptides
• They also may facilitate the selection of high-affinity antigenic peptides by allowing the dissociation of poorly fitting non-CLIP peptides to be exchanged for higher affinity peptides
• This peptide-exchange function of H2-M is essential for the intrathymic development of the repertoire of CD4+ T lymphocytes and the maturation of humoral and cell-mediated immune responses
• H2-M is believed to be expressed in all cells which express classical MHC class II molecules, including peripheral B lymphocytes, macrophages, dendritic cells, thymic cortical epithelium, and some tumor cell lines
• Its level of expression is at least partially controlled by CD74 (Ii), whereas its functional activity is negatively regulated by another non-classical MHC class II molecule, H2-O
• Host Species: Rat Clone: 2E5A Isotype: IgG1 κ Species Reactivity: Mouse Immunogen: Recombinant H2-DM protein Immunoprecipitation, Intracellular Staining