CD126 Mouse anti-Human, Biotin, Clone: M182, BD

Description

• Description The M182 antibody reacts with the a subunit of the human IL-6 Receptor (IL-6R α , aka, CD126)
• The human IL-6R α is an 80 kDa type I transmembrane glycoprotein, also known as B cell stimulatory factor-2 (BSF-2) receptor and IL-6 receptor
• The IL-6R α subunit associates with the 130-160 kDa gp130 subunit (IL-6 receptor ß chain, CD130), that is shared with the receptors for Leukemia Inhibitory Factor (LIF), Ciliary Neurotropic Factor (CNTF), Oncostatin M (OSM), IL-11, Cardiotropin 1 (CT-1) and possibly Neurotrophin-1/B Cell-Stimulating Factor 3 (NNT-1/BSF-3)
• The IL-6R α chain binds IL-6 with low affinity, however the association with CD130 stabilizes the IL-6/IL-6R α complex resulting in the formation of a high affinity complex
• The IL-6R ß chain mediates signal transduction
• IL-6R α s are expressed at high levels by activated and EBV-transformed B cells, plasma cells and myeloma cells and at lower levels by most leucocytes, epithelial cells, fibroblasts, hepatocytes and neural cells
• IL-6R α exists in soluble form in human serum
• The serum levels of soluble IL-6Raappear to elevate in pathological situations such as multiple myeloma, Grave's disease, juvenile chronic arthritis and HIV.10-13 The immunogen used to generate the M182 hybridoma was a mixture of U266, XG-1 (human myeloma cell line expressing membrane IL-6R) and BWD41 cells (murine thymoma cell line transfected with cDNA encoding the extracellular part of IL-6R α ).