BDB552593

CD3 Mouse anti-Human, BD IMag-DM, Clone: HIT3A, BD

Manufacturer: BD Biosciences

Select a Size

Pack Size SKU Availability Price
Each of 1 BDB552593-Each-of-1 In Stock ₹ 55,892.00

BDB552593 - Each of 1

₹ 55,892.00

In Stock

Quantity

1

Base Price: ₹ 55,892.00

GST (18%): ₹ 10,060.56

Total Price: ₹ 65,952.56

Antigen

CD3

Classification

Monoclonal

Conjugate

BD IMag-DM

Formulation

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Purification Method

Affinity Purified

Regulatory Status

RUO

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C

Applications

Flow Cytometry

Clone

HIT3A

Description

CD3E; T3E; TCRE; cd 3; cd-3; cd3; CD3-epsilon; 916

Host Species

Mouse

Quantity

5 mL

Research Discipline

Immunology

Target Species

Human

Isotype

IgG2a κ

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Description

  • BD IMag™ Anti-Human CD3 Particles DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces
  • These particles are optimized for the positive selection or depletion of CD3-bearing leukocytes using the BD IMagnet™
  • CD3 has been reported to be expressed on all mature T lymphocytes and on developing thymocytes
  • Peripheral Blood Mononuclear Cells (PBMC) are labeled with BD IMag™ anti-human CD3 Particles - DM according to the Magnetic Labeling Protocol
  • This labeled cell suspension is then placed within the magnetic field of the BD IMagnet™ (Cat
  • No
  • 552311)
  • Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction)
  • The tube is then removed from the magnetic field for resuspension of the positive fraction
  • The separation is repeated to increase the purity of the positive fraction
  • The magnetic separation steps are diagrammed in the Separation Flow Chart
  • After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications such as flow cytometry.