Bcl-2 Hamster anti-Mouse, Unlabeled, Clone: 3F11, BD

Description

• Bcl-2 is considered to be novel among proto-oncogenes because it blocks apoptosis (programmed cell death) in many cell types
• Apoptosis is an active form of cellular suicide that typically requires new RNA and protein synthesis and is associated with distinct morphological changes including cell shrinkage, cytoplasm membrane blebbing, nuclear fragmentation and DNA degradation
• The Bcl-2 gene was first found in t(14:18) containing follicular B-cell lymphomas
• A high proportion of these lymphomas contain t(14:18) chromosomal translocations involving the human Bcl-2 gene
• Translocation of Bcl-2 sequences from chromosome 18 onto the transcriptionally active immunoglobulin locus at chromosome band 14q32 in B-cells deregulates Bcl-2 gene expression, resulting in high levels of Bcl-2 mRNA and protein expression
• Because Bcl-2 blocks apoptosis it may contribute to tumorigenisis by prolonging cell survival rather than by accelerating the rate of cell proliferation
• Mouse Bcl-2 migrates at a reduced molecular weight of ∽26 kD
• This antibody recognizes a 26 kD band representing the mouse p26-Bcl-2 protein
• Additional minor bands at 27-31 kD and 18-21 kD may be visualized
• The 27-31 kD upper band may represent a larger isoform, whereas the 18-21 kD lower band may be an internal translation or proteolytic product
• 3F11 does not cross-react with human Bcl-2
• For detection of human Bcl-2, refer to clone 6C8 (Cat
• No
• 551051), or clone 4D7 (Cat
• No
• 554202).