DNA Topoisomerase I Mouse anti-Human, Unlabeled, Clone: C-21.2, BD

Description

• DNA damage may be caused by various environmental factors, including radiation, mutagenic chemicals and copy errors which occur during DNA replication
• The correction of DNA damage, so called “proofreading” functions of the cell, is achieved by numerous excision-repair enzymes
• Topoisomerases alter the helical structure of DNA by introducing the transient breaking and rejoining of DNA strands, allowing other excision-repair enzymes to correct DNA errors
• Topoisomerase I (Topo I) is a ubiquitous, soluble enzyme whose expression is fairly constant throughout the cell cycle
• The related enzyme, Topo II, is a primarily insoluble structural protein whose expression varies between cell types and during the cell cycle
• In addition to its role in DNA mismatch repair, Topo I displays kinase activity, phosphorylating serine-arginine rich (SR) splicing factors, and perhaps regulating gene expression by changing the splicing pattern of structural genes
• DNA Topo I migrates at a molecular weight of 100kDa in SDS-PAGE
• Clone C-21 recognizes human DNA Topoisomerase I
• The antibody is routinely tested by western blot analysis of A-431 cell lysates
• Host Species: Mouse Clone: C-21 Isotype: IgM Species Reactivity: Human Western Blotting