MGMT Mouse anti-Human, Unlabeled, Clone: MT5.1, BD

Description

• The repair of mismatched DNA is essential to maintaining the integrity of genetic information over time
• The important role played by DNA repair enzymes is emphasized by the fact that they are highly conserved from bacteria to yeast to mammals
• In bacteria, DNA repair involves the activity of repair enzymes including the MutL, MutH, and MutS proteins, as well as an enzyme originally identified in bacteria, and subsequently in all mammalian species, MGMT (methylguanine-DNA methyltransferase)
• Human MGMT is a 21kDa protein which protects cells from the mutagenic effect of alkylating agents, which add a methyl group to the O6 position of guanine in DNA, producing O6-methylguanine
• While MGMT is generally ubiquitously expressed in mammalian cells, the expression levels within cell and tissue types can be quite variable
• Certain tumor cell types have been identified which express neither MGMT protein or mRNA, despite the apparent normal expression of the MGMT gene
• The variable expression of MGMT may be a factor in the susceptibility of cells and tissues to the mutagenic effects of alkylating agents
• It has been suggested that methylation of cytosine in the MGMT gene and promoter region may correlate with the expression of the MGMT gene product
• MGMT is observed at 21kDa on SDS-PAGE
• Host Species: Mouse Clone: MT5.1 Isotype: IgG1 Cross Reactivity: Human Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blotting