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BDB560718

CD135 Rat anti-Mouse, APC, Clone: A2F10.1, BD

Manufacturer: BD Biosciences

Select a Size

Pack Size SKU Availability Price
Each of 1 BDB560718-Each-of-1 In Stock ₹ 17,693.20

BDB560718 - Each of 1

₹ 17,693.20

In Stock

Quantity

1

Base Price: ₹ 17,693.20

GST (18%): ₹ 3,184.776

Total Price: ₹ 20,877.976

Antigen

CD135

Classification

Monoclonal

Concentration

0.2mg/mL

Description

Flt3; Fms-like tyrosine kinase 3; FLT-3; FLK-2; Fetal liver kinase 2; Ly72

Host Species

Rat

Purification Method

Affinity Purified

Regulatory Status

RUO

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications

Flow Cytometry

Clone

A2F10.1

Conjugate

APC

Formulation

Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen

Mouse Flt-3 Transfected Cell Line

Quantity

50 μg

Research Discipline

Immunology

Target Species

Mouse

Isotype

IgG2a κ

Description

  • The A2F10 monoclonal antibody specifically binds to Flk-2/Flt3 (Ly-72, CD135), a receptor protein tyrosine kinase closely related to c-kit, c-fms, and PDGF Receptor of the immunoglobulin superfamily
  • The Flt3 message is detected in hematopoietic stem cells and primitive progenitor cells in fetal liver, adult bone marrow, and fetal and adult thymus, as well as brain, placenta, and testis; but it is absent in more mature hematopoietic cells
  • In flow cytometric analysis, the A2F10 antibody recognizes Flt3 -transfected Y3 cells (rat myeloma), but not the parent cell line in addition to recognizing early B lymphoid lineage cells in juvenile and adult bone marrow
  • A role for CD135 in the regulation of hematopoiesis is suggested by the observations that soluble Flk-2/Flt3 ligand can both stimulate proliferation of stem cell-enriched fetal liver, fetal thymus, and adult bone marrow populations and enhance their responses to other growth factors in vitro
  • In addition, injection of Flk-2/Flt3 ligand stimulates extramedullary hematopoiesis in the mouse spleen and accumulation of dendritic cells in the hematopoietic system
  • mAb A2F10.1 is reported to immunoprecipitate a 150-kDa surface protein from the murine myeloblast cell line M1, which naturally expresses CD135, and to inhibit the binding of Flk-2/Flt3 ligand to CD135.