IgD Mouse anti-Human, V500, Clone: IA6-2, BD

Description

• The IA6-2 monoclonal antibody specifically binds to the heavy chain of human Immunoglobulin D (IgD)
• IgD is a member of the immunoglobulin superfamily that exists in type 1-membrane (mIgD) and soluble glycoprotein forms
• mIgD is expressed on mature naοve B cells (along with membrane IgM) and serves as a B-cell receptor for antigen (BCR)
• In response to antigen binding, the mIgD BCR, in association with other signaling molecules including CD79a and CD79b, can transduce activating or tolerizing signals intracellularly into B lymphocytes
• When compensating dyes in this spectral range (such as Horizon™ V500 and AmCyan), the most accurate compensation can be obtained using single stained cellular controls
• Due to spectral differences between cells and beadsin this channel, using BD CompBeads can result in spillover errors for V500 and AmCyan reagents.Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended.Different V500 reagents (e.g
• CD4 vs
• CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents The antibody is conjugated to BD Horizon™ V500, which has been developed for use in multicolor flow cytometry experiments and isavailable exclusively from BD Biosciences
• It is excited by the Violet laser with an Ex max of 415nm and Em Max at 500 nm
• BD Horizon V500 conjugates emit at a similar wavelength to Amcyan yet exhibit reduced spillover into the FITC channel
• For more information on BD Horizon V500, visit bdbiosciences.com/colors
• When compensating dyes in this spectral range (such as Horizon™ V500 and AmCyan), the most accurate compensation can be obtained using single stained cellular controls
• Due to spectral differences between cells and beadsin this channel, using BD CompBeads can result in spillover errors for V500 and AmCyan reagents.Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended.Different V500 reagents (e.g
• CD4 vs
• CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents