BDB561946

CD8 Mouse anti-Human, PE-Cy5, Clone: HIT8A, BD

Manufacturer: Fischer Scientific

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Antigen

CD8

Classification

Monoclonal

Conjugate

PE-Cyanine5

Host Species

Mouse

Quantity

25 Tests

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications

Flow Cytometry

Clone

HIT8A

Formulation

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Purification Method

Affinity Purified

Regulatory Status

RUO

Target Species

Human

Isotype

IgG1 κ

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Description

  • Reacts with the α subunit (32kDa) of the two-chain complex
  • CD8 molecule binds to HLA class I molecules during interaction of CD8+ T cells with antigen-presenting cells or with target cells
  • CD8 is expressed on T cytotoxic/suppressor cell populations
  • HIT8a stains approximately 13 - 48% of peripheral blood lymphocytes and 80% of thymocytes, as well as a subset of NK cells
  • Clones HIT8a and RPA-T8 are not cross-blocking
  • This antibody is routinely tested by flow cytometric analysis
  • Other applications were tested at BD Biosciences Pharmingen during antibody development only
  • Host Species: Mouse Clone: HIT8a Isotype: IgG1 κ Species Reactivity: Human Flow Cytometry