CD4 Mouse anti-Human, Brilliant Violet 711, Clone: SK3, BD

Description

• The SK3 monoclonal antibody specifically binds to CD4
• The CD4 antigen is a 55 kDa type 1 transmembrane glycoprotein that belongs to the immunoglobulin superfamily
• It is expressed on T-helper/inducer lymphocytes and monocytes
• CD3+CD4+ T cells comprise approximately 28% to 58% of normal peripheral blood lymphocytes
• CD4 is also expressed on 80% to 95% of normal thymocytes
• The CD4 antigen is present in low density on the cell surface of monocytes and in the cytoplasm of monocytes and macrophages (CD3-CD4+)
• CD4 serves as a T cell coreceptor for MHC class II antigen recognition and as a receptor for the human immunodeficiency virus (HIV)
• The SK3 antibody inhibits HIV binding to CD4+ cells
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon™ BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy™5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.