CD314 (NKG2D) Mouse anti-Human, Brilliant Violet 650, Clone: 1D11, BD

Description

• The 1D11 monoclonal antibody specifically binds to NKG2D, a 42 kDa type II transmembrane glycoprotein that is also known as CD314 and KLRK1
• NKG2D is a member of the C-type lectin family and is expressed on human NK cells
• This activating receptor binds strongly to several ligands including MICA and MICB and ULBP-1, -2, and -3 proteins that are expressed by different target cell types
• Different from natural cytotoxicity receptor (NCR), NKG2D expression is not confined to NK cells
• It is also expressed on virtually all TCR γ/δ+ and CD8+TCR α/β+ T cells
• NKG2D functions as a triggering receptor involved in natural cytotoxicity mediated by normal NK cells against a variety of tumors or normal target cells
• Importantly, NKG2D can complement the role of NCR in tumor cell lysis
• Remarkably, the combined maskings of NCR and NKG2D can reportedly lead to a complete inhibition of NK-mediated lysis of all tumor or normal cells
• The 1D11 antibody can reportedly block or stimulate the function of NKG2D-positive cells
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will bespillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.