CD117 Mouse anti-Human, Brilliant Violet 650, Clone: 104D2, BD

Description

• The 104D2 monoclonal antibody specifically binds to human CD117, the receptor for stem cell factor (SCF)
• It selectively recognizes NIH- 3T3 cells transfected with human c-kit, the gene that codes for SCF-R
• The 104D2 antibody does not block the epitope that binds SCF
• In the bone marrow of humans and mice, SCF is expressed primarily on hematopoietic progenitor cells
• Lack of functional SCF or deficient SCF-R caused by mutations in the Sl and W loci, respectively, can result in severe anemia and a decrease in the number of primitive progenitor cells in mice
• Human hematopoietic progenitor cells can be recognized by their surface expression of CD34
• This cell population constitutes a small subset (1% to 5%) of bone marrow cells
• CD34+ cells contain a small subpopulation of primitive/non-committed progenitors, with the remaining fraction being cells committed to the various hematopoietic lineages
• SCF alone induces extensive proliferation of erythroid-committed progenitor cells (CD34lo CD71hi CD64-)
• On primitive (CD34hi CD38lo CD50+) and granulo-monocytic (CD34+ CD64+) progenitor cells, SCF synergistically enhances the effects of other cytokines, the strongest of which are on the primitive progenitor cells
• In addition, SCF promotes survival of primitive progenitors in the absence of proliferation
• The receptor is highly expressed at similar levels on all of the three mentioned CD34+ cell subsets, whereas B-lymphoid committed progenitor cells (CD34+ CD19+) express low levels of SCF-R
• Among CD34- bone marrow cells, only a small number of cells (mostly erythroid) express the receptor
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will bespillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.