CD8A Rat anti-Mouse, Brilliant Violet 421, Clone: 53-6.7, BD

Description

• The 53-6.7 antibody monoclonal antibody specifically binds to the 38 kDa α and 34 kDa α chains of the CD8 differentiation antigen (Ly-2 or Lyt-2) of all mouse strains tested
• The CD8 α and α chains (CD8a) form heterodimers with the CD8 β chain (CD8b, Ly-3, or Lyt-3) on the surface of most thymocytes
• A subpopulation of mature T lymphocytes (i.e., MHC class I-restricted T cells, including most T suppressor/cytotoxic cells) expresses almost exclusively the CD8 αβ heterodimer (the α α chains arise from alternatively spliced messengers of a single CD8a gene
• The longer α form associates with p56 [lck] via a CXCP motif in its cytoplasmic domain, which it shares with CD4, but not with CD8b
• The truncated α chain is unable to associate with p56 [lck], and it may function to attenuate the CD8-mediated costimulatory signal during intrathymic T-cell maturation
• In vivo and in vitro treatment with 53-6.7 mAb has reportedly been effective at depleting CD8+ peripheral T lymphocytes
• The 53-6.7 antibody has also been reported to cross-reaact with CD8 α - and α -like polypeptides on subsets of thymic and peripheral lymphocytes in the Egyptian toad, Bufo regularis
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.