CD86 Rat anti-Mouse, BUV395, Clone: GL1, BD

Description

• The GL1 antibody has been reported to react with the B7-2 (CD86) costimulatory molecule expressed on a broad spectrum of leukocytes, including B lymphocytes, T lymphocytes, thioglycollate-induced peritoneal macrophages, dendritic cells and astrocytes
• CD86 is expressed at low levels by freshly explanted peripheral B and T cells, and its expression is substantially increased by a variety of T cell- and B cell-specific stimuli with a peak expression after 18-42 hours of culture
• In contrast to most naive CD4+ T cells, memory CD4+ T cells express B7-2, both at the mRNA and protein level
• CD86, a ligand for CD28 and CD152 (CTLA-4), is one of the accessory molecules that plays an important role in T cell-B cell costimulatory interactions
• It has been shown to be involved in immunoglobulin class-switching and triggering of mouse NK cell-mediated cytotoxicity
• CD80 (B7-1) is an alternate ligand for CD28 and CD152 (CTLA-4)
• GL1 antibody reportedly blocks MLR and stimulation of T cells by natural antigen-presenting cells
• In addition, a mixture of anti-B7-1 and anti B7-2 (GL1) mAbs reportedly inhibits the in vitro interaction of CTLA-4 with its ligand and the in vivo priming of cytotoxic T lymphocytes
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors
• With an Ex Max at 348nm and an Em Max at 395nm, BD Horizon BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.