CD25 Mouse anti-Human, BB515, Clone: 2A3, BD

Description

• The 2A3 monoclonal antibody specifically binds to human CD25, the low-affinity alpha subunit of the Interleukin-2 Receptor (IL- 2Rα)
• CD25 associates with CD122 (IL-2Rβ chain) and CD132 (common γchain or γc) to f orm the high-affinity signal-transducing IL-2R complex
• CD25 is expressed by subsets of thymocytes and peripheral blood lymphocytes including CD4+CD25+ regulatory T cells and memory T cells
• CD25 antigen density increases on activated T cells including phytohemagglutinin (PHA)-, concanavalin A (Con A)-, and CD3-activated T lymphocytes
• High levels of CD25 can be expressed by T lymphocytes from mixed lymphocyte cultures and by human T-lymphocyte leukemia virus (HTLV)-infected T-lymphocyte leukemia lines, for example, HUT-102
• CD25 can also be expressed by activated B cells and macrophages
• Recombinant IL-2 blocks the binding of the 2A3 antibody to PHA-activated T lymphocytes
• The antibody was conjugated to BD Horizon™ BB515 which was developed exclusively by BD Biosciences
• With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g
• 530/30-nm filter)
• This dye provides a much brighter alternative to FITC with less spillover into the PE detector.