BDB565013

Ig Goat anti-Rat, Brilliant Violet 421, Polyclonal, BD

Manufacturer: BD Biosciences

Select a Size

Pack Size SKU Availability Price
Each of 1 BDB565013-Each-of-1 In Stock ₹ 19,580.00

BDB565013 - Each of 1

₹ 19,580.00

In Stock

Quantity

1

Base Price: ₹ 19,580.00

GST (18%): ₹ 3,524.40

Total Price: ₹ 23,104.40

Antigen

Ig

Classification

Polyclonal

Conjugate

Brilliant Violet 421

Host Species

Goat

Quantity

50 μg

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications

Flow Cytometry

Concentration

0.2mg/mL

Formulation

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Purification Method

Affinity Purified

Regulatory Status

RUO

Target Species

Rat

Isotype

Ig

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Description

  • BD Horizon™ BV421 Goat Anti-Rat Ig is intended to be a second-step reagent for immunofluorescent staining of cells pre-stained with Rat Ig primary antibodies
  • It also stains rat B cells and has little reactivity with rat non-B cells or mouse splenocytes
  • As a second step, it is reactive with rat IgG and IgM monoclonal antibodies although a weaker signal may be detected when the primary antibody has a rat IgM or IgG2b isotype due to its adsorption with mouse Ig
  • It has weak crossreactivity detectable by flow cytometry with some, but not all, hamster immunoglobulins
  • The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
  • BD Horizon BV421 has an Ex Max at 407nm and Em Max at 421 nm
  • The use of a mounting reagent (eg, ProLong™ Gold) is highly recommended to maximize the photostability of BV421
  • For epifluorescence microscopes with broad spectrum excitation sources, the recommended excitation and emission filters are 392/23nm and 430/24nm bandpass filters, respectively
  • For specific multicolor imaging applications, the exact filter configurations should be optimized by the end user
  • For additional instrument/filter configuration information, please visit http://www.bdbiosciences.com/research/cellularimaging
  • For flow cytometry, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter).