CD36 Mouse anti-Mouse, BB515, Clone: CRF D-2712, BD

Description

• The CRF D-2712 monoclonal antibody specifically binds to mouse and rat CD36, an 88-kDa glycoprotein member of the class B scavenger receptor family
• CD36 is a multifunctional receptor that has a variety of ligands and is expressed on many cell types
• Consequently, it is known by several names, including macrophage scavenger receptor, platelet glycoprotein IV, and fatty acid translocase
• Cells that express CD36 include platelets, megakaryocytes, monocytes/macrophages, dendritic cells, erythroid precursors, microvascular endothelia, skeletal, cardiac, and smooth muscle cells, adipocytes, and epithelia of retina, breast, and intestine
• This multi-ligand receptor functions in the clearance of apoptotic cells and abnormal erythrocytes (in malaria and sickle-cell anemia), the transport of long-chain fatty acids and oxidized low-density lipoproteins, the regulation of angiogenesis by mediating the effects of thrombospondin-1, and the mediation of coronary vasoconstriction
• Crosslinking of cell-surface CD36 with plate-bound CRF D-2712 antibody promotes internalization of photoreceptor outer segment fragments by cultured retinal pigment epithelium
• The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences
• With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g
• 530/30-nm filter)
• This dye provides a much brighter alternative to FITC with less spillover into the PE detector.