CD279 Hamster anti-Mouse, APC-R700, Clone: J43, BD

Description

• The J43 monoclonal antibody specifically binds to CD279 (PD-1), a 50-55-kDa glycoprotein encoded by the Pdcd1 gene of the CD28 family of the Ig superfamily
• The expression of Pdcd1 mRNA and PD-1 protein is tightly regulated
• PD-1 is transiently expressed on CD4-CD8thymocytes, it is upregulated on some cell lines upon induction of apoptosis, it is induced on thymocytes and splenic T and B lymphocytes after stimulation through their antigen receptors, and it is induced on activated myeloid cells
• In addition, Pdcd1 mRNA is transiently expressed in developing B lymphocytes at the pro-B-cell stage
• The presence of an ITIM ( yrosine-based I nhibitory M otif) on PD-1's intracytoplasmic region and the development of splenomegaly and breakdown of peripheral tolerance in PD-1[-/-] mice suggest that PD-1 may be involved in the negative regulation of immune responses
• The PD-1 ligands, B7-H1 (also known as PD-L1) and B7-DC (PD-L2), are members of the B7 family of the Ig superfamily
• The J43 antibody blocks the binding of PD-1 to its two ligands
• This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor™ 700
• APC-R700 excites and emits at similar wavelengths to Alexa Fluor™ 700 yet exhibits significantly improved brightness
• This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor™ (eg, 730/45-nm filter).