TREX1 Mouse anti-Mouse, Unlabeled, Clone: 29, BD

Description

• DNA replication, repair, and recombination requires the excision of nucleotides from the DNA 3′ termini
• Many 3′ to 5′ exonucleases have been identified which catalyze the excision of monophosphates from the 3′ termini of DNA
• TREX1 and TREX2 are 3′ to 5′ exonucleases that contain three conserved exonuclease active site motifs (EASM) that may produce exonuclease activity
• TREX1 and TREX2 are most closely related to the proofreading exonucleases of the bacterial replicative DNA polymerases and the RNase T enzymes
• Recombinant expression of TREX1 and TREX2 demonstrates that they have exonuclease activity when oligonucleotide is present
• TREX1 shows the greatest exonuclease activity with partial duplex DNA, and no activity with single-stranded RNA or an RNA-DNA partial duplex
• In addition, reconstitution of TREX1 with DNA polymerase β and DNA ligase III-XRCC1 facilitates accurate rejoining of a 3′ mismatched base residue at a single-strand break
• Thus, TREX1 and TREX2 are 3′ to 5′ exonucleases that may be important for excision of nucleotides during DNA replication, repair, and recombination
• Host Species: Mouse Clone: 29 Isotype: IgG1 Species Reactivity [for Features Main]: Human Immunogen: Mouse TRE1 aa
• 82-179 Immunofluorescence, Western Blotting