CD16 Mouse anti-Baboon, Human, Rhesus, Brilliant Ultraviolet 737, Clone: 3G8, BD Horizon™

Description

• The 3G8 monoclonal antibody specifically binds to the 50-65 kDa transmembrane form of the IgG Fc Receptor (Fc γRIII), a human NK cell-associated antigen
• CD16 is expressed on NK cells as well as macrophages and granulocytes
• Reports indicate that CD16 plays a role in signal transduction and NK cell activation
• The 3G8 antibody blocks the binding of soluble immune complexes to granulocytes
• The 3G8 antibody is reported (Vossebeld et al ., 1997) to increase intracellular calcium levels in human neutrophils by interacting with both Fc γRIIa and Fc γRIIIb molecules
• This antibody has also been reported to induce homotypic neutrophil aggregation
• The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm
• BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter
• Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor™ 700-like dyes (e.g., 712/20-nm filter)
• Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV737 reagents
• Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended
• Different BUV737 reagents (e.g., CD4 vs
• CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.