CD127 Mouse anti-Human, Brilliant Ultraviolet 737, Clone: HIL-7R-M21, BD Horizon™

Description

• The hIL-7R-M21 monoclonal antibody specifically binds to the 60-90 kDa glycoprotein, CD127
• CD127 is also known as the IL-7 receptor alpha (IL-7R α ) subunit
• The IL-7 receptor complex is a heterodimer composed of CD127 and the common gamma chain ( γc, CD132), shared by other cytokine receptors (IL-2R, IL-4R, IL-9R, IL-15R, and IL-21R)
• CD127 is expressed on thymocytes, T- and B-cell progenitors, mature T cells, and some lymphoid and myeloid cells
• In vitro experiments show the expression of CD127 is down-regulated following T cell activation
• Studies indicate that the IL-7 Receptor plays an important role in the proliferation and differentiation of mature T cells
• Recently, it has been shown that low surface expression of CD127, in combination with intermediate to high surface expression of CD25, the α chain of the IL-2 receptor complex, can distinguish between human regulatory and conventional CD4+ T cells in human adult and cord blood, lymph nodes and thymus
• Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV737 reagents
• Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended
• Different BUV737 reagents (e.g., CD4 vs
• CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.