CD95 Mouse anti-Human, BUV395, Clone: DX2, BD Optibuild™

Description

• The DX2 monoclonal antibody specifically binds to the human Fas antigen (also called APO-1)
• This 45 kDa transmembrane cell surface molecule was designated as CD95 at the Fifth HLDA Workshop
• Fas is a member of the TNF-receptor superfamily and is also known as Tumor necrosis factor receptor superfamily member 6 (TNFRSF6)
• It is expressed on a variety of normal and neoplastic cells including activated T and B lymphocytes and some undifferentiated thymocytes
• The Fas/CD95 antigen is a polypeptide that plays a role in the programmed sequence of events leading to cell death, termed apoptosis
• The DX2 clone specifically reacts with murine L cells, murine L1210 leukemia cells and murine P815 mastocytoma cells transfected with human Fas cDNA but not with untransfected parental cell lines
• Crosslinking CD95 with DX2 antibody delivers an apoptotic signal indicating that DX2 recognizes a functional epitope of the CD95 antigen
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348nm and an Em Max at 395nm, BD Horizon BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.