CD29 Hamster anti-Mouse, Brilliant Violet 605, Clone: HM β1-1, BD Optibuild™

Description

• The HM β 1-1 monoclonal antibody specifically binds to the 130-kDa integrin β 1 chain (CD29)
• CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin- α chains
• With α 1 through α 6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with α v (CD51), it forms α α 8, and α 9 chains in non-lymphoid tissues
• As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, epithelia, and oocytes
• This hamster mAb to a mouse leukocyte antigen has been observed to crossreact with similar populations of rat leukocytes
• Source of the immunogen was purified mouse VLA-4 ( α 4β 1, CD49d/CD29)
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.