CD102 Rat anti-Mouse, Brilliant Violet 650, Clone: 3C4(mIC2/4), BD Optibuild™

Description

• The 3C4 (mIC2/4) monoclonal antibody specifically binds to the mouse ICAM-2 (CD102) cell surface glycoprotein, a ligand for LFA-1
• CD102 is constitutively expressed on endothelial cells, T and B lymphocytes, and alveolar walls
• It is also expressed on a variety of leukocyte cell lines
• CD102 does not appear to be involved in the development of hematopoietic cells
• In a model for allergic asthma, endothelial CD102 mediates the transmigration of eosinophils (but not lymphocytes, monocytes, or macrophages) into the airway lumen
• The 3C4 (mIC2/4) antibody blocks interactions between ICAM-2 and LFA-1
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.