IgM Mouse anti-Human, Brilliant Violet 650, Clone: G20-127, BD Optibuild™

Description

• IgM is an important component in the first line of defense against foreign pathogens, but may also play a role in autoimmune diseases
• IgM monomers consist of two light and two heavy chains
• Unlike the heavy chain of an IgG antibody which contains 3 constant Ig domains, the µ heavy chain of IgM contains 4 constant Ig domains
• Five IgM monomers complex with a small polypeptide (J-chain) to form pentameric IgM that can be found in human plasma
• In an immune response, the binding of IgM to a cell surface antigen enables C1q to activate interactions with downstream components in the classical complement pathway
• Mature B lymphocytes express IgM
• The G20-127 monoclonal antibody binds to the heavy chain of human IgM
• The G20-127 antibody is not thought to react with other immunoglobulin heavy chain isotypes
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.