CD184 Rat anti-Mouse, Brilliant Violet 711, Clone: 2B11/CXCR4, BD Optibuild™

Description

• The 2B11/CXCR4 monoclonal antibody specifically binds to mouse CD184, which is also known as the C-X-C Chemokine Receptor type 4 , CXCR4
• CXCR4 (previously known as Fusin and LESTR), is a seven-transmembrane, G-protein-coupled receptor
• It is the specific receptor for the CXC chemokine, SDF-1/CXCL12
• Mouse CXCR4 shows 91% homology at the amino acid level with human CXCR4
• CXCR4 is widely expressed by hematopoietic and non-hematopoietic cell types including neutrophils, monocytes, T cells, B cells, CD34-positive progenitor cells, endothelial cells, neurons and astrocytes
• Human CXCR4 is used by T-tropic HIV-1 as a co-receptor for viral entry
• The mouse Cxcr4 gene has been mapped to chromosome 1
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.