CD138 Rat anti-Mouse, Brilliant Violet 786, Clone: 281-2, BD Optibuild™

Description

• The 281-2 monoclonal antibody specifically binds to the core protein of CD138 (Syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules
• Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization
• It is also expressed on B lymphocytes at specific stages during their differentiation: precursor B cells in the bone marrow and antibody-secreting cells, including plasma cells, but not mature peripheral B cells
• It is thus implicated in mediating B cell-matrix interactions
• CD138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational modifications
• The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein
• Furthermore, the mAb detects the Syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase
• The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm
• BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).