BDB742472

H-2Ld/H-2Db Mouse anti-Mouse, Brilliant Violet 711, Clone: 28-14-8, BD Optibuild™

Manufacturer: BD Biosciences

CAS Number: 28-14-8

Select a Size

Pack Size SKU Availability Price
Each of 1 BDB742472-Each-of-1 In Stock ₹ 35,422.00

BDB742472 - Each of 1

₹ 35,422.00

In Stock

Quantity

1

Base Price: ₹ 35,422.00

GST (18%): ₹ 6,375.96

Total Price: ₹ 41,797.96

Antigen

H-2Ld/H-2Db

Clone

28-14-8

Conjugate

Brilliant Violet 711

Gene Alias

H-2Db/H-2Ld, H2-Db/H2-Ld, Histocompatibility-2 Db/Ld

Immunogen

C3H.SW mouse splenocytes

Quantity

50 μg

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Classification

Monoclonal

Concentration

0.2mg/mL

Formulation

Aqueous buffered solution containing ≤0.09% sodium azide.

Host Species

Mouse

Purification Method

Affinity Purified

Regulatory Status

RUO

Target Species

Mouse

Isotype

IgG2a κ

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Description

  • The 28-14-8 antibody reacts with theα3 domain of the H-2D[b] MHC class I alloantigen
  • The antibody binds to H-2D[b] in the presence or absence of theβ2 microglobulin chain
  • It cross-reacts with theα3 domain of H-2L[d], but not with K[d] or D[d], and with H-2D[q] and/or L[q]
  • Reactivity with haplotypes k, f, p, r, /and s /has not been observed
  • mAb 28-14-8 has been shown to block H-2L[d]- specific and H- 2L[d]-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes (CTL), but it does not block recognition of H-2L[d] positive target cells by Ly-6G2-positive NK cells
  • The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
  • This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
  • BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
  • Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
  • However, the spillover can be corrected through compensation as with any other dye combination
  • BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
  • This dye offers a very bright choice for the violet laser
  • Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
  • BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.