BDB742763
CD21 Mouse anti-Human, Brilliant Violet 711, Clone: 1048, BD Optibuild™
Manufacturer: BD Biosciences
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | BDB742763-Each-of-1 | In Stock | ₹ 49,528.50 |
BDB742763 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 49,528.50
GST (18%): ₹ 8,915.13
Total Price: ₹ 58,443.63
Antigen
CD21
Clone
1048
Conjugate
Brilliant Violet 711
Gene Alias
CR2, Complement receptor type 2, C3DR, EBV-R, Epstein-Barr virus receptor
Immunogen
Human CR2 Recombinant Protein
Quantity
50 μg
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Classification
Monoclonal
Concentration
0.2mg/mL
Formulation
Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species
Mouse
Purification Method
Affinity Purified
Regulatory Status
RUO
Target Species
Human
Isotype
IgG1 κ
Related Products
Description
- CD21 is a 145 kDa transmembrane glycoprotein that is expressed on mature B cells, follicular dendritic cells, a subset of immature thymocytes and on some epithelial cells
- CD21 is also known as the C3d Receptor, Complement Receptor 2 (CR2), and the Epstein-Barr Virus receptor, (EBV-R)
- CD21 is part of a large signal-transduction complex that also includes CD19, CD81 and Leu13
- CR2 serves as a receptor for the activation fragments of complement component C3, i.e
- C3d, iC3d, and C3dg and C3d-bound antigens and plays a central role in the immune response by amplifying B-cell activation and proliferation
- CR2 is also the receptor for EBV by binding its surface glycoprotein gp350/220
- Anti-human CR2 hybridomas were generated from Cr2-/-mice (lacking the cr2 gene, that encodes CR2 and CR1 in mice) immunized with the recombinant protein representing the first two amino-terminal short consensus repeats (SRC) of human CR2, SCR1-2
- Both the C3d and EBV binding sites of hu CR2 were localized to these sites
- Clone 1048 has been reported to specifically react with CR2-expressing cells and to inhibit CR2 interactions with C3 fragments or the EBV surface protein, gp350/220
- The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
- This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
- BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
- Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
- However, the spillover can be corrected through compensation as with any other dye combination
- BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
- This dye offers a very bright choice for the violet laser
- Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
- BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.