GARP Mouse anti-Human, Brilliant Violet 650, Clone: 7B11 (also known as CMSSC-7B11), BD Optibuild™

Description

• The 7B11 (also known as CMSSC-7B11) monoclonal antibody specifically binds to human GARP (Glycoprotein A repetitions predominant)
• The LRRC32 /(Leucine rich repeat containing 32) gene encodes the 662 amino acid-residue, 80 kDa transmembrane GARP glycoprotein that has an extracellular region composed primarily of 20 leucine-rich repeats
• GARP is specifically expressed on Treg cells activated through the T cell receptor (TCR)
• Ectopic expression of GARP in human naοve T cells inhibited their proliferation and cytokine secretion upon TCR activation
• Remarkably, GARP over-expression in naοve T cells induced expression of FoxP3 and endowed them with a partial suppressive function
• The extracellular, but not the cytoplasmic region, of GARP was necessary for these functions
• GARP serves as a receptor for latent TGF-beta which may play a role in the suppressive action of Treg cells
• GARP is also expressed on platelets and other tissues, however the function on these cells is not known
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.