BDB742817
MAdCAM-1 Rat anti-Mouse, Brilliant Violet 786, Clone: MECA-367, BD Optibuild™
Manufacturer: BD Biosciences
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | BDB742817-Each-of-1 | In Stock | ₹ 35,600.00 |
BDB742817 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 35,600.00
GST (18%): ₹ 6,408.00
Total Price: ₹ 42,008.00
Antigen
MAdCAM-1
Clone
MECA-367
Conjugate
Brilliant Violet 786
Gene Alias
Madcam1, MADCA, mucosal vascular addressin cell adhesion molecule 1
Immunogen
Mouse endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes.
Quantity
50 μg
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Classification
Monoclonal
Concentration
0.2mg/mL
Formulation
Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species
Rat
Purification Method
Affinity Purified
Regulatory Status
RUO
Target Species
Mouse
Isotype
IgG2a κ
Related Products
Description
- The MECA-367 monoclonal antibody specifically recognizes mucosal vascular addressin MAdCAM-1
- In the fetus and neonate, MAdCAM-1 is the predominant vascular addressin on the high endothelial venules (HEV) of peripheral lymph nodes
- In adult mice, MAdCAM-1 is preferentially expressed in mucosal lymphoid tissues and lamina propria; it is also expressed on sinus-lining cells in the spleen
- MAdCAM-1 expression is upregulated on the HEV of peripheral lymph nodes in adult NOD mice4 and is involved in the development of diabetes and insulitis
- Furthermore, there is evidence that IFN-γcan induce MAdCAM-1 expression in non-mucosal sites in adult mice
- MAdCAM-1 is a predominant ligand for integrinα4β7, a lymphocyte mucosal homing receptor, and a facultative ligand for CD62L (L-selectin)
- MECA-367 mAb binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro /and lymphocyte homing to Peyer's patch HEV in vivo /
- Source of the immunogen was endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes
- The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes
- This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm
- BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter)
- BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm
- BV786 offers a bright choice for the 6th detector off the violet laser.