BDB743720
CD253 Mouse anti-Human, Brilliant Violet 605, Clone: RIK-2, BD Optibuild™
Manufacturer: BD Biosciences
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | BDB743720-Each-of-1 | In Stock | ₹ 49,528.50 |
BDB743720 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 49,528.50
GST (18%): ₹ 8,915.13
Total Price: ₹ 58,443.63
Antigen
CD253
Clone
RIK-2
Conjugate
Brilliant Violet 605
Gene Alias
TNFSF10, TRAIL, APO-2L, TL2
Immunogen
Human TRAIL Transfected Cell Line
Quantity
50 μg
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Classification
Monoclonal
Concentration
0.2mg/mL
Formulation
Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species
Mouse
Purification Method
Affinity Purified
Regulatory Status
RUO
Target Species
Human
Isotype
IgG1
Related Products
Description
- The RIK-2 monoclonal antibody specifically binds to CD253, also known as TRAIL (TNF-Related Apoptosis-Inducing Ligand) and Apo2 ligand (APO-2L)
- CD253 is a member of the Tumor Necrosis Factor Superfamily and is encoded by the TNFSF10 gene
- CD253 is a type II membrane protein that may be expressed as a soluble as well as full-length, cell surface-associated protein
- Both surface and soluble forms of TRAIL rapidly induce apoptosis in a wide range of tumor cell lines but not normal tissue
- TRAIL is expressed by activated T cells, NK cells, dendritic cells, monocytes and a variety of non-lymphoid cells
- TRAIL can bind to and exert apoptosis through DR4 (TRAIL-R1) and DR5 (TRAIL-R2) receptors
- It can also bind to decoy receptors, including DcR1/TRID/TRAIL-R3 and DcR2/TRUNDD/TRAIL-R4, and possibly OPG/TNFRSF11B, which may serve to regulate TRAIL activity
- TRAIL has been shown to be involved in T cell-mediated cytotoxicity, but its mechanism of action remains to be fully elucidated
- The RIK-2 clone was selected based on its ability to block TRAIL-mediated cytotoxic activity
- This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
- With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
- BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
- Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
- BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
- BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
- These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
- Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.