M006T02Y04A

CD62L (L-Selectin) Monoclonal Antibody (MEL-14), NovaFluor™ Yellow 660, eBioscience™, Invitrogen™

Manufacturer: LIFE TECHNOLOGIES

Select a Size

Pack Size SKU Availability Price
Each of 1 M006T02Y04A-Each-of-1 In Stock ₹ 16,287.00

M006T02Y04A - Each of 1

₹ 16,287.00

In Stock

Quantity

1

Base Price: ₹ 16,287.00

GST (18%): ₹ 2,931.66

Total Price: ₹ 19,218.66

Antigen

CD62L (L-Selectin)

Classification

Monoclonal

Concentration

0.1 mg/mL

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.

P18337

Host Species

Rat

Quantity

25 μg

Primary or Secondary

Primary

Target Species

Mouse

Format

Liquid

Applications

Flow Cytometry

Clone

MEL-14

Conjugate

NovaFluor Yellow 660

Gene

SELL

Gene Alias

A.11, AI528707, CD62 antigen-like family member L, CD62L, gp90-MEL, hLHRc, LAM1, LAM-1, LECAM1, LECAM-1, LEU8, Leu-8, Leukocyte adhesion molecule 1, leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, LNHR, LSEL, L-selectin, Ly22, Ly-22, LYAM1, Lyam-1, Ly-m22, Lymph node homing receptor, lymphocyte adhesion molecule 1, lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, pln homing receptor, PLNHR, SELE, selectin E (endothelial adhesion molecule 1), selectin L, selectin L (lymphocyte adhesion molecule 1), selectin, lymphocyte, Sell, TQ1

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

20343

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Isotype

IgG2a κ

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Description

  • The MEL-14 mAb reacts with mouse CD62L, a 76 kDa member of the selectin family
  • CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1
  • These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues
  • The MEL-14 antibody has been reported for use in flow cytometric analysis
  • Our internal testing shows that NovaFluor Yellow 660 non-specifically stains B cells in SJL mice
  • Non-specific staining has not been observed in BALB/c or C57BL/6 mice
  • Other strains have not been tested
  • See the Antibody Testing Data for an example of this strain-dependent difference
  • The MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes
  • This can be used at less than or equal to 0.2 ug per test
  • A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer Excitation: 552 nm; Emission: 663 nm; Laser: 561 nm (Yellow) Laser CD62L (L-Selectin) Monoclonal antibody specifically detects CD62L (L-Selectin) in Mouse samples
  • It is validated for Flow Cytometry