TRIM28 Monoclonal Antibody (20C1), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MA12023-Each-of-1 | In Stock | ₹ 47,837.50 |
MA12023 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 47,837.50
GST (18%): ₹ 8,610.75
Total Price: ₹ 56,448.25
Antigen
TRIM28
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
PBS with 1mg/mL BSA and 0.05% sodium azide
Gene Accession No.
Q13263, Q62318
Gene Symbols
TRIM28
Immunogen
Recombinant protein corresponding to residues 60-383 of the human TIF1 beta protein.
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human, Mouse
Product Type
Antibody
Isotype
IgG1
Applications
ChIP Assay, Gel Shift, Immunocytochemistry, Immunoprecipitation, Western Blot
Clone
20C1
Conjugate
Unconjugated
Gene
TRIM28
Gene Alias
AA408787; E3 SUMO-protein ligase TRIM28; KAP1; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-A-interacting protein; KRAB-associated protein 1; KRAB-interacting p; KRAB-interacting protein 1; Krip1; KRIP-1; MommeD9; Nuclear corepressor KAP-1; PPP1R157; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; RNF96; TF1B; tif1 beta; Tif1b; Tif1beta; TIF1-beta; Transcription intermediary factor 1-beta; transcriptional intermediary factor 1, beta; transcriptional intermediary factor 1-beta; Trim28; tripartite motif; tripartite motif containing 28; tripartite motif protein 28; tripartite motif-containing 28; tripartite motif-containing protein 28
Host Species
Mouse
Purification Method
Protein G
Regulatory Status
RUO
Gene ID (Entrez)
10155, 21849
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Form
Liquid
Related Products
Description
- MA1-2023 detects human and mouse TIF1 beta
- MA1-2023 has been used successfully in Western blot, and immunofluorescence procedures
- By Western blot this antibody detects ∽100 kDa protein representing TIF1 beta in gamma irradiated HeLa cell lysate
- In immunofluorescence procedures, MA1-2023 recognizes TIF1 beta in gamma irradiated HeLa cells
- The MA1-2023 immunogen is recombinant protein corresponding to residues 60-383 of the human TIF1 beta protein
- The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors
- There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome
- These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1)
- KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger
- KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression
- KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1)
- KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression
- Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.