MA182787
Phosphotyrosine Monoclonal Antibody (pY20), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MA182787-Each-of-1 | In Stock | ₹ 40,228.00 |
MA182787 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 40,228.00
GST (18%): ₹ 7,241.04
Total Price: ₹ 47,469.04
Antigen
Phosphotyrosine
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
BBS with <0.1% sodium azide; pH 8.0
Host Species
Mouse
Purification Method
Affinity chromatography
Regulatory Status
RUO
Target Species
Chemical
Product Type
Antibody
Isotype
IgG2b
Applications
ELISA, Immunocytochemistry, Western Blot
Clone
pY20
Conjugate
Unconjugated
Gene Alias
Phosphotyrosine; pTyr; pY
Immunogen
Proprietary Immunogen.
Quantity
500 μg
Primary or Secondary
Primary
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
Target Molecule
Chemical
Related Products
Description
- For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM sodium orthovanadate should be added to the sample buffer
- Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background
- Mouse anti Phosphotyrosine antibody, clone PY20 recognizes phosphotyrosine, enabling the detection, characterisation and isolation of proteins containing phosphorylated tyrosine residues
- The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years
- While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research
- Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function
- The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.