MABC168925

GFP, Mouse anti-All, Clone: 2B6, Millipore Sigma™

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 MABC168925-Each-of-1 In Stock ₹ 17,216.16

MABC168925 - Each of 1

₹ 17,216.16

In Stock

Quantity

1

Base Price: ₹ 17,216.16

GST (18%): ₹ 3,098.909

Total Price: ₹ 20,315.069

Antigen

GFP

Classification

Monoclonal

Conjugate

Unconjugated

Formulation

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Gene Symbols

GFP

Immunogen

His-tagged recombinant jellyfish Green fluorescent protein.

Quantity

25 μg

Research Discipline

Secondary & Control Antibodies

Test Specificity

Clone 2B6 is a mouse monoclonal antibody that detects GFP-conjugated to most proteins.

Content And Storage

Stable for 1 year at 2-8°C from date of receipt.

Isotype

IgG1 κ

Applications

Immunocytochemistry, Western Blot

Clone

2B6

Dilution

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected GFP in HEK293T cells stabely transfected with GFP-tagged Aquaporin 4.

Gene Alias

Green fluorescent protein

Host Species

Mouse

Purification Method

Protein G purified

Regulatory Status

RUO

Primary or Secondary

Primary

Target Species

All species

Form

Purified

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Description

  • Anti-GFP, clone 2B6, Cat
  • No
  • MABC1689, is a mouse monoclonal antibody that detects GFP-conjugated to most proteins and has been tested for use in Immunocytochemistry and Western Blotting
  • Green fluorescent protein (UniProt: P42212) is encoded by the GFP gene in Aequorea victoria (Jellyfish)
  • Green fluorescence protein (GFP) is a 27 kDa protein from jellyfish that serves as an energy-transfer acceptor
  • It transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer and it fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin
  • GFP has an absorption max at 395 nm and the emission peaks at 507 to 510 nm
  • However, the exact value of emission max depends on the environment of the chromophore
  • GFP is an important tool in cell biology research that displays stability under fixation conditions and is suitable for a variety of applications, such as tracing cell lineage tracer, reporter of gene expression, or exploring protein-protein interactions.