MilliporeSigma™ E. coli anti-poly-ADP-ribose binding reagent, Polyclonal,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABE1031MI-Each-of-1 | In Stock | ₹ 50,817.22 |
MABE1031MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 50,817.22
GST (18%): ₹ 9,147.10
Total Price: ₹ 59,964.32
Antigen
poly-ADP-ribose binding reagent
Classification
Polyclonal
Host Species
E. coli
Quantity
200 μL
Research Discipline
Epigenetics and Nuclear Function
Target Species
Human, Mouse
Form
Purified
Applications
Dot Blot
Formulation
Purified fromE. coliby Ni-NTA agarose. Supplied in buffer containing 10mM Tris pH 7.5, 0.2M NaCl, 10% Glycerol, 10mM Imidazole, 1mM PMSF, 1mM β-Mercaptoethanol, 10% glycerol without preservatives.
Purification Method
Ni-NTA agarose
Regulatory Status
RUO
Primary or Secondary
Primary
Content And Storage
Stable for one year at -80°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
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Description
- Specifically detects poly-ADP-ribose binding reagent in Human, Mouse samples, and it is validated for Dot Blot Anti-poly-ADP-ribose binding reagent, is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta™(DE3)pLysS strain of E
- coli
- Anti-poly-ADP-ribose binding reagent is useful for the affinity detection of oligo- and poly-ADP-ribosylated (PARylated) proteins on membranes or on fixed cells in a manner similar to antibody-based Western blot, dot blot, and immunocytochemistry applications
- The rabbit Fc tag allows visualization of the binding/labeling with conjugated anti-rabbit secondary antibodies
- The Fc tag also allows Anti-poly-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications.