Histone H1, Mouse anti-Drosophila, Clone: 1G6, Millipore Sigma™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABE188525-Each-of-1 | In Stock | ₹ 17,118.26 |
MABE188525 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,118.26
GST (18%): ₹ 3,081.287
Total Price: ₹ 20,199.547
Antigen
Histone H1
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
His 1
Immunogen
GST-tagged recombinant fragment corresponding to 148 amino acids from the C-terminal half of Drosophila Histone H1.
Quantity
25 μg
Research Discipline
Epigenetics & Nuclear Function
Gene ID (Entrez)
NP_001027286
Target Species
Drosophila
Form
Purified
Applications
ChIP Assay, Immunoprecipitation, Western Blot
Clone
1G6
Dilution
ChIP-seq Analysis: A representative lot detected Histone H1 in ChIP-seq applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19). Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H1 in Immunoprecipitation applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated Histone H1 in Chromatin Immunoprecipitation applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).
Gene Alias
H1
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 1G6 is a mouse monoclonal antibody that detects Histone H1 in Drosophila species.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Anti-Histone H1, clone 1G6 Antibody, Cat
- No
- MABE1885, is a highly specific mouse monoclonal antibody that targets Drosophila Histone H1 and has been tested for use in Chromatin Immunoprecipitation (ChIP), ChIP-seq, Immunoprecipitation, and Western Blotting
- Histone H1 (UniProt: P02255; also known as H1) is encoded by the His 1 gene (Gene ID: 318854) in Drosophila species
- Histone H1 is a linker histone that is involved in the organization and maintenance of chromatin higher order structure and plays an active role in the control of DNA replication and cell proliferation
- The linker histone H1 family members are a key component of chromatin and bind to the nucleosomal core particle around the DNA entry and exit sites
- Histone H1 is essential for the condensation of nucleosome chains into higher-order structures
- In metazoans, H1 histones are smaller in size with about 200 amino acids
- They have a short N-terminal tail, a central globular domain, and a long basic C-terminal tail
- The central globular domain is shown to be highly conserved among all H1 subtypes
- Manipulating levels of H1 histone can lead to both up- and down-regulation of specific genes
- Depletion of H1 results in a massively altered chromosome structure with loss of chromosome banding
- In Drosophila, a 20% reduction in H1 content leads to cessation of growth beyond the larval stage
- Histone H1 undergoes phosphorylation mainly in the tail region, especially the C-terminal tail, where several {(S/T)-P-X-(K/R)} motifs are located that are recognized by cyclin-dependent kinases (CDKs)
- Phosphorylation levels are reported to be lowest during the G1 phase of the cell cycle and increase during S phase and reach maximum levels in mitosis
- (Ref.: Hergeth, SP., and Schneider, R (2015)
- EMBO Rep
- 16(11); 1439-1453).