Klotho, Rat anti-Human, Clone: KL-115, Millipore Sigma™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABN2398100-Each-of-1 | In Stock | ₹ 42,969.20 |
MABN2398100 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 42,969.20
GST (18%): ₹ 7,734.456
Total Price: ₹ 50,703.656
Antigen
Klotho
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Rat monoclonal antibody in ascites with 0.1% sodium azide with 50% glycerol.
Gene Symbols
KL
Immunogen
GST-tagged recombinant fragment corresponding to 207 amino acids from the extracellular domain from the N-terminal region of human Klotho.
Quantity
100 μL
Research Discipline
Neuroscience
Gene ID (Entrez)
NP_004786
Target Species
Human
Isotype
IgG2a κ
Applications
Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Clone
KL-115
Dilution
Western Blotting Analysis: A represetative lot detected Klotho in Western Blotting applications (Maltare, A., et. al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7).Immunocytochemistry Analysis: A represetative lot detected Klotho in Immunocytochemistry applications (Maltare, A., et. al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7).Immunoprecipitation Analysis: A represetative lot immunoprecipitated Klotho in Immunoprecipitation applications (Maltare, A., et. al. (2014). Monoclon Antib Immunodiagn Immunoother. 33(6):420-7).
Gene Alias
EC: 3.2.1.31;KL
Host Species
Rat
Purification Method
Unpurified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone KL-115 is a rat monoclonal antibody that specifically detects human Klotho. It targets an epitope with in 107 amino acids from the KL1 domain from the N-terminal region.
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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Description
- Anti-Klotho, clone KL-115, Cat
- No
- MABN2398, is a highly specific rat monoclonal antibody that targets klotho and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting
- Klotho (UniProt: Q9UEF7; also known as EC: 3.2.1.31) is encoded by the KL gene (Gene ID:9365) in human
- Klotho is a homodimeric, highly N-glycosylated, single-pass type I membrane protein that is present in kidney, placenta, small intestine, and prostate tissue
- In kidney it is predominantly present in cortical renal tubules
- A soluble secreted form (isoform 2, ∽ 64 kDa) is present in serum and cerebrospinal fluid
- The Klotho peptide generated by cleavage of the membrane-bound isoform (Isoform 1) is considered as an anti-aging circulating hormone that is reported to extend life span by inhibiting insulin/IGF1 signaling
- It also acts as a cofactor for interaction of FGF23 with FGF R1, which negatively regulates the activity of 1-alpha-hydroxylase, the rate limiting enzyme in the synthesis of 1,25(OH)2D3
- Klotho is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to generate the mature form that has an extracellular domain (aa 31-981), a short transmembrane domain (aa 982-1002), and a short cytoplasmic tail (aa 1003-1012)
- Deficiency of Klotho is reported to shorten life-span in animal models with a myriad of disorders associated with old age, such as arteriosclerosis, osteoporosis, and skin atrophy
- Klotho deficient mice also display severe hyperphosphatemia and soft tissue calcification
- On the contrary, over-expression of Klotho is shown to extend life-span in murine models by about 30% with side effects, such as mild insulin resistance and enhanced resistance to oxidative stress
- Defects in KL gene has been linked to chronic renal failure complications.