MilliporeSigma™ Platelet Factor 4, Mouse, Unlabeled, Clone: 197.2,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABS1255MI-Each-of-1 | In Stock | ₹ 43,318.08 |
MABS1255MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 43,318.08
GST (18%): ₹ 7,797.254
Total Price: ₹ 51,115.334
Antigen
Platelet Factor 4
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified mouse IgG1 in buffer containing PBS without preservatives.
Gene Accession No.
P02776
Immunogen
Purified human platelet PF4 (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
Quantity
100 μg
Research Discipline
Signaling
Gene ID (Entrez)
NP_002610
Target Species
Human
Form
Purified
Applications
Flow Cytometry, Immunocytochemistry, Western Blot
Clone
197.2
Conjugate
Unconjugated
Gene
PF4, CXCL4, SCYB4
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 197.2 targets a linear epitope sensitive to heparin binding, but insensitive to reducing agent or denaturation by SDS (Xiao, Z., et al. (2008). Blood. 112(4):1091-1100).
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype
IgG1 κ
Related Products
Description
- Platelet factor 4 (UniProt P02776; also known as C-X-C motif chemokine 4, Iroplact, Oncostatin-A, PF-4) is encoded by the PF4 (also known as CXCL4, SCYB4) gene (Gene ID 5196) in human
- Platelet factor 4 (PF4) is a small CXC chemokine family cytokine released from alpha-granules of activated platelets during platelet aggregation to promote blood coagulation
- PF4 binds heparin with high affinity (Kd ∽4-20 nM) via its heparin-binding domain (a.a
- 92-98) and neutralizes the anti-thrombin activity of heparin-like molecules
- PF4 is chemotactic for neutrophils, fibroblasts and monocytes, and interacts with the chemokine receptor CXCR3 splice variant CXCR3B
- In individuals suffering from heparin-induced thrombocytopenia (HIT), PF4-heparin complexes can trigger an immune response and the generation of anti-PF4 antibodies, resulting in the foramtion of macromolecular antibody-heparin-PF4 immune complexes that bind Fc RIIa (CD32a) on platelets and induce platelet activation
- Platelet activation in turn results in more PF4 release into circulation, enhanced immune-complex formation, exaggerated platelet activation, and cell clearance
- PF4 is synthesized with a signal peptide sequence (a.a
- 1-31) that is removed posttranslationally to produce the mature PF4 (a.a
- 32-101), additional proteolytic cleavage yields the PF4 short form (a.a
- 48-101).