OTUD3, Mouse anti-Human, Clone: 12A6.1, Millipore Sigma™
Manufacturer: MilliporeSigma
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABS181925-Each-of-1 | In Stock | ₹ 17,118.26 |
MABS181925 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,118.26
GST (18%): ₹ 3,081.287
Total Price: ₹ 20,199.547
Antigen
OTUD3
Classification
Monoclonal
Conjugate
Unconjugated
Gene Alias
OTU domain-containing protein 3;EC 3.4.19.12
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Human
Form
Purified
Applications
Western Blot
Clone
12A6.1
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
OTUD3;KIAA0459
Immunogen
GST/His-tagged recombinant fragment corresponding to 115 amino acids from the C-terminal half of human OTU domain-containing protein 3.
Quantity
25 μg
Research Discipline
Signaling
Test Specificity
Clone 12A6.1 detects OTU domain-containing protein 3 in human cells. It iargets an epitope within 115 amino acids from the C-terminal region.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Anti-OTUD3, clone 12A6.1, Cat
- No
- MABS1819, is a mouse monoclonal antibody that detects OTUD3 in human cells and has been tested for use in Western Blotting
- OTU domain-containing protein 3 (UniProt: Q5T2D3; also known as EC: 3.4.19.12, OTUD3) is encoded by the OTUD3 (also known as KIAA0459) gene (Gene ID: 23252) in human
- OTUD3 is a cytoplasmic deubiquitinating enzyme that hydrolyzes Lys-6- and Lys-11-linked polyubiquitin and is reported to hydrolyze heterotypic (mixed and branched) and homotypic chains
- It has an OUT domain (aa 65-189) and a UBA-like domain (aa 230-270)
- Its specificity is given by the S1' ubiquitin-binding site within the OTU domain composed of the Cys-, His- and Variable-loops
- The UBA-like domain has no effect on ubiquitin hydrolysis
- It displays a thiol-dependent ubiquitin-specific protease activity
- It is reported to regulate PTEN stability and suppresses tumorigenesis
- OTUD3 transgenic mice exhibit higher levels of the PTEN and are shown to be less prone to tumorigenesis
- OTUD3 is believed to regulate PTEN in a manner, which is dependent on its DUB activity
- The catalytically inactive mutant C76A does not upregulate PTEN and may even have a downregulating effect
- It can remove Lys-6, Lys-11, Lys-27, and Lys-48 types of ubiquitin chain on PTEN
- (Ref.: Yuan, L., et al (2015)
- Nat
- Cell Biol
- 17(9); 1169-1181).