MT-ND4, Mouse anti-Human, Clone: 9E4-2D8, Millipore Sigma™
Manufacturer: MilliporeSigma
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABS199425-Each-of-1 | In Stock | ₹ 17,118.26 |
MABS199425 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,118.26
GST (18%): ₹ 3,081.287
Total Price: ₹ 20,199.547
Antigen
MT-ND4
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG2a in buffer containing HEPES-Buffered Saline (150 mM NaCl, 15 mM HEPES, pH 7.2) with 0.02% sodium azide.
Gene Symbols
MT-ND4;MTND4;NADH4;ND4
Immunogen
KLH-conjugated linear peptide from the C-terminal region.
Quantity
25 μg
Research Discipline
Signaling
Test Specificity
Clone 9E4-2D8 detects NADH-ubiquinone oxidoreductase chain 4 in mitochodria isolated from human cells.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG2a
Applications
Western Blot
Clone
9E4-2D8
Dilution
Western Blotting Analysis: 4μg/mL from a representative lot detected MT-ND4 in mitochondria from Human Neonatal Dermal Fibroblasts and mitochondria from Human neonatal dermal fibroblasts depleted of mtDNA. (Courtesy of Michael F. Marusich, Ph.D., mAbDx, Inc., Eugene, OR, USA ).
Gene Alias
NADH-ubiquinone oxidoreductase chain 4;EC: 1.6.5.3;NADH dehydrogenase subunit 4
Host Species
Mouse
Purification Method
Protein L
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Human
Form
Purified
Related Products
Description
- Anti-MT-ND4, clone 9E4-2D8, Cat
- No
- MABS1994,is a mouse monoclonal antibody that detects NADH-ubiquinone oxidoreductase chain 4 and has been tested for use in Western Blotting
- NADH-ubiquinone oxidoreductase chain 4 (UniProt: P03905; also known as EC: 1.6.5.3, NADH dehydrogenase subunit 4, MT-ND4) is encoded by the MT-ND4 (also known as MTND4, NADH4, ND4) gene (Gene ID: 4538) in human
- MT-ND4 is an inner mitochondrial membrane protein that belongs to the complex I subunit 4 family
- It is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I)
- The complex couples the oxidation of NADH and the reduction of ubiquinone (Coenzyme Q10), to the generation of a proton gradient, which is then used for ATP synthesis
- The complex can be dissociated into two main sub-complexes, corresponding to the "ankle" of the boot, and the "foot" of the boot
- The ankle is thought to protrude from the membrane so as to be predominantly in the aqueous phase on the matrix side
- It contains the binding site for NAD(H), and the input electron transfer chain
- The foot (hydrophobic) is membrane bound and contains a catalytic site at which ubiquinone is reduced
- Defects in MT-ND4 gene are known to cause Leber hereditary optic neuropathy (LHON) that is characterized by acute or subacute loss of central vision due to optic nerve dysfunction
- LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes
- Other diseases associated with MT-ND4 gene mutations are age-related macular degeneration, mesial temporal lobe epilepsy, and cystic fibrosis.