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MABS83125UG

PI3 Kinase, Mouse anti-Mouse, Human, Clone: 1D5.1, Millipore Sigma™

Manufacturer: MilliporeSigma

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Pack Size SKU Availability Price
Each of 1 MABS83125UG-Each-of-1 In Stock ₹ 17,118.26

MABS83125UG - Each of 1

₹ 17,118.26

In Stock

Quantity

1

Base Price: ₹ 17,118.26

GST (18%): ₹ 3,081.287

Total Price: ₹ 20,199.547

Antigen

PI3 Kinase

Classification

Monoclonal

Conjugate

Unconjugated

Formulation

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Gene Symbols

PIK3R1;GRB1

Immunogen

GST/His-tagged recombinant fragment corresponding to 77 amino acids from the N-terminal region of the p85 regulatory subunit of human PI 3-kinase alpha.

Quantity

25 μg

Research Discipline

Signaling

Test Specificity

Clone 1D5.1 detects p85 subunit of human and murine PI-3 kinase alpha. It targets an epitope within 77 amino acids from the N-terminal region.

Content And Storage

Stable for 1 year at 2-8°C from date of receipt.

Isotype

IgG2b κ

Applications

Western Blot

Clone

1D5.1

Dilution

Western Blotting Analysis: 0.5μg/mL from a representative lot detected PI3 Kinase, p85, N-SH3 in 10μg of human cerebral cortex tissue lysate.

Gene Alias

Phosphatidylinositol 3-kinase regulatory subunit alpha;Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha;PI3-kinase regulatory subunit alpha;PtdIns-3-kinase regulatory subunit p85-alpha;PI3-kinase subunit p85-alpha;PI3K regulatory subunit alpha;PtdIns-3-kinase regulatory subunit alpha

Host Species

Mouse

Purification Method

Protein G purified

Regulatory Status

RUO

Primary or Secondary

Primary

Target Species

Mouse, Human

Form

Purified

Description

  • Anti-PI3 Kinase, p85, N-SH3, clone 1D5.1, Cat
  • No
  • MABS831, is a mouse monoclonal antibody that detects human and murine p85 subunit of PI3 kinase
  • It has been tested for use in Western Blotting
  • Phosphatidylinositol 3-kinase regulatory subunit alpha (UniProt: P27986; also known as Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha) is encoded by the PIK3R1 (also known as GRB1) gene (Gene ID: 5295) in human
  • PI3K regulatory subunit alpha catalyzes the phosphorylation of PI(4,5)P2 inositol ring at the 3 position to generate PI(3,4,5)P3, a potent second messenger that mediates survival signaling and insulin action
  • PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit
  • Following ligand binding, tyrosine phosphorylation of growth factor receptors creates docking sites for p85 binding (through its SH2 domains), and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane
  • PI3 kinase alpha plays a role in insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues
  • Mutations in PIK3R1 gene are known to be associated with insulin resistance.