Desmoplakin, Mouse anti-Human, Clone: 20B6, Millipore Sigma™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABT149225-Each-of-1 | In Stock | ₹ 17,118.26 |
MABT149225 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,118.26
GST (18%): ₹ 3,081.287
Total Price: ₹ 20,199.547
Antigen
Desmoplakin
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
DSP
Immunogen
His-tagged recombinant fragment corresponding to 863 amino acids from the C-terminal half of human Desmoplakin.
Quantity
25 μg
Research Discipline
Cell Structure
Gene ID (Entrez)
NP_001008844
Target Species
Human
Form
Purified
Applications
Immunocytochemistry, Western Blot
Clone
20B6
Dilution
Western Blotting Analysis: A representative lot detected Desmoplakin in Western Blotting applications (Sobolik-Delmaire, T., et. al. (2006). J Biol Chem. 281(25):16962-70; Hall, C., et. al. (2009). Cell Commun Adhes. 16(1-3):15-27; Roberts, B.J., et. al. (2011). Exp Cell Res. 317(20):2814-22).Immunocytochemistry Analysis: A representative lot detected Desmoplakin in Immunocytochemistry applications (Roberts, B.J., et. al. (2011). Exp Cell Res. 317(20):2814-22; Sobolik-Delmaire, T., et. al. (2006). J Biol Chem. 281(25):16962-70; Roberts, B.J., et. al. (2016). J Biol Chem. 291(48):24857-24865).
Gene Alias
DP;250/210 kDa paraneoplastic pemphigus antigen
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 20B6 detects human Desmoplankin. It targets an epitope with in the C-terminal half.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Anti-Desmoplakin, clone 20B6, Cat
- No
- MABT1492, is a mouse monoclonal antibody that detects human Desmoplakin and has been tested for use in Immunocytochemistry and Western Blotting
- Desmoplakin (UniProt: P15924; also known as DP, 250/210 kDa paraneoplastic pemphigus antigen) is encoded by the DSP gene (Gene ID:1832) in human
- Desmoplakin is a major high molecular weight homodimeric protein that is involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes, which are prominent adhesive junctions present between many epithelial cells and cardiomyocytes
- Desmoplakin is the most abundant of the desmosomal proteins and plays a key role in tissue morphogenesis and maintaining the integrity of tissue
- Desmoplakin is composed of an N-terminal plakin domain, a central alpha-helical coiled-coil rod domain, and a C-terminal intermediate filament-binding domain
- The N-terminal domain is required for desmosome association and the C-terminal binds to desmin
- Three isoforms for Desmoplakin have been reported that are produced by alternative splicing
- Desmoplakin 2 and 3 lack most of the central rod domain
- Desmoplakin 1 is the dominant isoform in the cardiac tissue
- Desmoplakin is phosphorylated by protein kinase A on serine 2849 and this phosphorylation affects its association with epidermal, simple cytokeratins, and VIM intermediate filaments
- Mutations in DSP gene have been linked to DCWHK (Cardiomyopathy, dilated, with woolly hair and keratoderma) that is characterized by a generalized striate keratoderma particularly affecting the palmoplantar epidermis, woolly hair, and dilated left ventricular cardiomyopathy
- Mice deficient in Desmoplakin perish around the time of implantation (E6.5) and show fewer desmosomes.